| 高产腈水解酶Alcaligenes faecalis ULA4菌株的诱变筛选 |
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| 引用本文： | 徐建明,柳志强,徐建妙,张金峰,郑裕国.高产腈水解酶Alcaligenes faecalis ULA4菌株的诱变筛选[J].微生物学杂志,2011,31(5):45-48. |
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| 作者姓名： | 徐建明 柳志强 徐建妙 张金峰 郑裕国 |
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| 作者单位： | 浙江工业大学生物工程研究所,浙江杭州,310014 |
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| 摘 要： | 应用紫外-氯化锂和低能离子束复合诱变的方法,对1株本实验室筛选得到并保藏的腈水解酶产生菌Alcaligenes faecalis ZJB09133进行诱变育种,筛选到高产菌株UL44,其腈水解酶酶活达到74.6 U/g,较出发.菌株酶活提高124.5％.得到的高产菌株UL44经过5次传代,其遗传稳定性良好.
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| 关 键 词： | 粪产碱杆菌 腈水解酶 亚氨基二乙腈 亚氨基二乙酸 诱变 |
Mutation Breeding of Nitrilase High-Yielding Alcaligenes faecalis Strain UL44 |
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| XU Jian-ming,LIU Zhi-qiang,XU Jian-miao,ZHANG Jin-feng,ZHENG Yu-guo.Mutation Breeding of Nitrilase High-Yielding Alcaligenes faecalis Strain UL44[J].Journal of Microbiology,2011,31(5):45-48. |
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| Authors: | XU Jian-ming LIU Zhi-qiang XU Jian-miao ZHANG Jin-feng ZHENG Yu-guo |
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| Institution: | XU Jian-ming,LIU Zhi-qiang,XU Jian-miao,ZHANG Jin-feng,ZHENG Yu-guo(Inst.of Bioengin.,Zhejiang Uni.of Technol.,Hangzhou 310014) |
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| Abstract: | A nitrilase producer Alcaligenes faecalis strain ZJB09133 screened and preserved in the lab was carried out composite mutation with UV-LiCl and low-energy ion.A high yielding mutant UL44 was screened with the nitrilase activity as high as 74.6 U/g,increased by 124.5% higher than the starter strain.The obtained UL44 strain proclaimed genetically stable after passing five rounds of generation. |
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| Keywords: | Alcaligenes faecalis nitrilase iminodiacetonitrile iminodiacetic acid mutation |
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