| 温度诱导双水相体系分离芦荟多糖的分配行为研究 |
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| 引用本文： | 李文君,李芬芳,邢健敏.温度诱导双水相体系分离芦荟多糖的分配行为研究[J].天然产物研究与开发,2011,23(6):1088-1090,1126. |
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| 作者姓名： | 李文君 李芬芳 邢健敏 |
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| 作者单位： | 中南大学化学化工学院,长沙,410083 |
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| 基金项目： | 国家自然基金项目，湖南省重点基金项目，长沙市科技计划项目 |
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| 摘 要： | 本文研究了芦荟多糖在温度诱导双水相体系中的分配行为,考察了Triton-114的浓度、温度、酸度、盐的浓度等因素对芦荟多糖分配行为的影响。结果表明,芦荟多糖趋于分配在水相,当Triton-114浓度为4%,pH=3,温度50℃时,芦荟多糖在水相中的回收率达到最大,多糖的含量也由原来的68.39%增加到75.63%。实验还表明,无机盐对芦荟多糖的分配行为具有很大的影响。
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| 关 键 词： | 温度诱导 芦荟多糖 双水相 |
Partitioning Behaviour of Aloe Polysaccharides by Temperature-Induced Aqueous Two-Phase System |
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| LI Wen-jun,LI Fen-fang,XING Jian-min.Partitioning Behaviour of Aloe Polysaccharides by Temperature-Induced Aqueous Two-Phase System[J].Natural Product Research and Development,2011,23(6):1088-1090,1126. |
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| Authors: | LI Wen-jun LI Fen-fang XING Jian-min |
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| Institution: | LI Wen-jun,LI Fen-fang,XING Jian-min School of Chemistry and Chemical Engineering,Central South University,Changsha 410083,China |
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| Abstract: | In this paper,the partitioning behavior of Aloe polysaccharides was investigated with TritonX-114 temperature-induced aqueous two-phase system.The effects of different factors including the concentration of Triton X-114,temperature,pH,and concentration of inorganic salt were investigated in details.The results showed that Aloe polysaccharides mostly accumulated in the aqueous phase.The optimal condition consisted of adding 4% (w/w) Triton X-114 and 0.3 mol/L inorganic salt,pH 3.0 and 50 ℃ under which the highest recovery rate was obtained and the content of Aloe polysaccharides increased from 68.39% to 75.63%.In addition,it was demonstrated that the inorganic salt had a great effect on the partitioning behavior of Aloe polysaccharides. |
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| Keywords: | temperature-induced aloe polysaccharides aqueous two-phase |
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