| HPLC示差折光法测定藻油中DHA的含量 |
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| 引用本文： | 肖小年,侯小晶,易醒. HPLC示差折光法测定藻油中DHA的含量[J]. 天然产物研究与开发, 2011, 23(6): 1110-1112 |
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| 作者姓名： | 肖小年 侯小晶 易醒 |
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| 作者单位： | 1. 南昌大学食品科学与技术国家重点实验室,中德联合研究院,南昌330047
2. 江西中德食品工程中心,南昌,330047 |
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| 摘 要： | 首次采用HPLC示差折光法测定藻油中DHA的含量。色谱柱为Eclipse XDB-C18(Analytical 4.6×250mm,5μm),流动相为乙腈∶四氢呋喃∶水(含0.4%HAC)=77∶3∶20,柱温为30℃,流速为1.0 mL/min。该条件下DHA在0.259～6.225 mg/mL内呈良好的线性关系,相关系数R2=0.9995,平均回收率为101.15%,RSD为1.98%(n=6)。该方法可靠、简便、重现性好,可作为藻油中DHA的定量方法。
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| 关 键 词： | 高效液相色谱法(HPLC) 示差折光检测器 藻油 二十二碳六烯酸(DHA) 测定 |
Determination of DHA in Algae Oil by HPLC with Differential Refractive Index Detector |
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| XIAO Xiao-nian,HOU Xiao-jing,YI Xing. Determination of DHA in Algae Oil by HPLC with Differential Refractive Index Detector[J]. Natural Product Research and Development, 2011, 23(6): 1110-1112 |
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| Authors: | XIAO Xiao-nian HOU Xiao-jing YI Xing |
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| Affiliation: | XIAO Xiao-nian1,HOU Xiao-jing1,YI Xing21 State Key Laboratory of Food Science and Technolodge,Sino-German Joint Research Institute,Nanchang University,Nanchang 330047,China,2Jiangxi Sino-German Food Engineering Center |
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| Abstract: | The content of DHA in algae oil was firstly determined by HPLC with differential refractive index detector.The sample was analyzed in a Eclipse XDB-C18(Analytical 4.6 × 250 mm,5 μm) with acetonitrile ∶ tetrahydrofuran ∶ water(containing 0.4% HAC) = 77∶ 3∶ 20 as the mobile phase,the column temperature was fixed at 30 ℃ and the flow rate was 1.0 mL/min.The standard curves of DHA presented the good linear correlation in the range of 0.259～6.225 mg/mL(R2 = 0.9995).The average recovery rate of DHA was 101.15% and the RSD(n = 6) was 1.98% under this con-dition.The results showed that HPLC method was reliable and simple with good reproducibility for determining DHA in algae oil. |
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| Keywords: | HPLC differential refractive index detector algae oil docosahexaenoic acid(DHA) determination |
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