A radioimmunoassay for quantifying carbonic anhydrase isozymes in crude lysates |
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| Authors: | Verle E. Headings Richard E. Tashian |
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| Affiliation: | (1) Department of Human Genetics, University of Michigan Medical School, Ann Arbor, Michigan;(2) Present address: Medical Genetics Unit, Howard University College of Medicine, Box 101, Washington, D.C. |
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| Abstract: | A radioimmunoassay was developed for quantifying each of the two genetically distinct forms of primate carbonic anhydrase, carbonic anhydrases I and II, in unpurified lysates. Under the given experimental conditions, the assay is capable of detecting a minimum of 0.025  g of carbonic anhydrase I and 0.005 g of carbonic anhydrase II. There is approximately 98% reproducibility upon repeated assays of a given hemolysate.Supported by NIH training grant 5-TO1-GM-71-11 and NIH research grant 1-PO1-GM-15419-02.NIH postdoctoral trainee. |
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