Comparative Analysis of Eubacterial DNA Polymerase Ⅲ Alpha Subunits |
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作者姓名: | Xiao-Qian Zhao Jian-Fei Hu Jun Yu |
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作者单位: | [1]Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China [2]Graduate School of Chinese Academy of Sciences, Beijing 100049, China [3]CoUege of Life Sciences, Peking University, Beijing100871, China [4]James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310008,China |
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摘 要: | DNA polymerase Ⅲ is one of the five eubacterial DNA polymerases that is responsible for the replication of DNA duplex. Among the ten subunits of the DNA polymerase Ⅲ core enzyme, the alpha subunit catalyzes the reaction for polymerizing both DNA strands. In this study, we extracted genomic sequences of the alpha subunit from 159 sequenced eubacterial genomes, and carried out sequence- based phylogenetic and structural analyses. We found that all eubacterial genomes have one or more alpha subunits, which form either homodimers or heterodimers. Phylogenetic and domain structural analyses as well as copy number variations of the alpha subunit in each bacterium indicate the classification of alpha subunit into four basic groups: polC, dnaE1, dnaE2, and dnaE3. This classification is of essence in genome composition analysis. We also consolidated the naming convention to avoid further confusion in gene annotations.
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关 键 词: | 真细菌 DNA聚合酶Ⅲ α亚基 基因组 氨基酸序列 比较分析 |
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