Expressed sequence tags and their application for plant research |
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Authors: | Email author" target="_blank">Agnieszka?KisielEmail author Jan?Podkowi ski |
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Institution: | (1) Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Pozna, Poland |
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Abstract: | Expressed Sequence Tags (ESTs) are short, usually unedited sequences obtained by single-pass sequencing of cDNA clones from
any cDNA library. Analyzing and comparing ESTs can provide information on gene expression, function and evolution. Large-scale
EST sequencing has become an attractive alternative to plant genome sequencing. Currently, plant EST collections comprise
over 3.8 million sequences from about 200 species. They have proved to be a valuable tool for gene discovery and plant metabolism
analysis. Several plant-specific EST databases have been created which provide access to sequence data and bioinformatics-based
tools for data mining. Searching EST collections allows pre-selection of genes for preparing cDNA arrays, targeted to bring
maximum information on specialized processes, like stress response, symbiotic nitrogen fixation etc. Also, ESt-based molecular
markers such as SNP, SSR, and indels are fast developing tools for breeders and researchers. |
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Keywords: | cDNA arrays EST functional genomics gene discovery gene mapping |
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