Bioremediation of Vegetable Oil and Grease from Polluted Wastewater Using a Sand Biofilm System |
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Authors: | El-Masry Mohamed H El-Bestawy Ebtesam El-Adl Nawal I |
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Institution: | (1) Department of Biotechnology, Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt;(2) Department of Environmental Studies, Institute of Graduate Studies & Research, Alexandria University, Alexandria, Egypt Tel.:;(3) Quality Control Lab, Extracted Oils and Derivatives Company, Alexandria, Egypt |
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Abstract: | Pseudomonas sp. (L1), P. diminuta(L2) were among eight bacterial strains isolated from vegetable grease and oil-contaminated industrial wastewater, four of
which only were found to have the ability to degrade oil and grease. They were identified and investigated for oil and grease
degradation either individually or in combinations in previous unpublished work by the authors. Since the combination M1 (Pseudomonas sp. andP. diminuta) produced the highest degradative activity, it was used in the present study in a biofilm sand filter system for vegetable
oil and grease removal. This system was tested either as one unit or two units in sequence where different flow rates (30,
50, 100 ml/h) were applied compared to a control unit(s). Results showed that both biofilm systems reduced oily wastewater,
even in cases of high degree of pollution (fat, oil & grease (FOG), 7535 ppm; biochemical oxygen demand (BOD5), 525 ppm; chemical oxygen demand (COD), 1660 ppm). Results also showed a removal of FOG with efficiency at 100%; BOD5 at 95.9% and COD at 96%, at 50 ml/h flow rate using one unit of biofilm system. On using two units in sequence, a complete
removal of FOG, BOD5 and COD with efficiency 100%, at flow rate 100 ml/h was achieved. In conclusion, the previous biofilm results indicated the
efficiency of such a system in treating oily polluted wastewater (vegetable oil origin) on the basis of bacterial isolates
being used, the optimum flow rate, and the number of biofilm units used in sequence to obtain the highest removal capacity
of such a system.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Biofilm bioremediation grease oil vegetable |
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