Interaction between the nitrate respiratory system of Escherichia coli K12 and the nitrogen fixation genes of Klebsiella pneumoniae. |
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Authors: | M L Skotnicki B G Rolfe |
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Affiliation: | Department of Genetics, Research School of Biological Sciences, The Australian National University, Canberra, A.C.T. 2601, Australia |
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Abstract: | Hybrids were constructed between K12 ? mutants defective in nitrate respiration and an F′ plasmid carrying nitrogen fixation genes from . Examination of these hybrids showed that expression of Kp+ genes does not require a functional nitrate respiratory system, but that nitrate reductase and nitrogenase do share some Mo-processing functions. For nitrate repression of nitrogenase activity, reduction of nitrate to nitrite is not necessary, but the Mo-X cofactor encoded by genes is essential. Nitrate probably inhibits nitrogen fixation by affecting the membrane relationship of the nitrate and fumarate reduction systems such that the membrane cannot be energized for nitrogenase activity. |
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