首页 | 本学科首页   官方微博 | 高级检索  
     

四种松毛虫不同地理种群遗传多样性的等位酶分析
引用本文:南宫自艳,高宝嘉,刘军侠,杨君. 四种松毛虫不同地理种群遗传多样性的等位酶分析[J]. 昆虫学报, 2008, 51(4): 417-423
作者姓名:南宫自艳  高宝嘉  刘军侠  杨君
作者单位:1. 河北农业大学林学院,河北保定,071001
2. 河北农业大学植保学院,河北保定,071001
摘    要:【目的】采用等位酶电泳技术对中国松毛虫属Dendrolimus 4种共9个地理种群进行遗传多样性和遗传分化研究。【方法】对6种等位酶系统乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、乙醇脱氢酶(ADH)、甲酸脱氢酶(FDH)、谷氨酸脱氢酶(GDH)进行聚丙烯酰胺凝胶电泳分析。【结果】在4种松毛虫9个地理居群中共检测到10个基因位点,其中4个位点为多态位点,检测到17个等位基因; 种群总体水平多态位点比率P=40%,平均有效基因数A=1.700,平均期望杂合度He=0.151,种群平均遗传距离为0.001~0.285; 其中马尾松毛虫指名亚种Dendrolimu punctatus Walker 6个居群的遗传分化度Fst=0.265,基因流Nm=0.692。4种松毛虫之间遗传关系最近的是落叶松毛虫D. superans Butler和马尾松毛虫的地理亚种赤松毛虫D. punctatus spectabilis Butler,遗传关系最远的是落叶松毛虫D. superans Butler和云南松毛虫D. houi Lajonquiere。【结论】 马尾松毛虫居群间遗传分化程度较大,基因交流较少,遗传漂变已经成为导致该物种种群分化的主要原因之一;遗传距离与地理距离存在一定相关性。

关 键 词:松毛虫属  地理种群  遗传多样性  等位酶  中国  

Genetic diversity of geographical populations of four Dendrolimus species (Lepidoptera:Lasiocampidae)in China based on allozyme analysis
NANGONG Zi-Yan,GAO Bao-Jia,LIU Jun-Xia,YANG Jun. Genetic diversity of geographical populations of four Dendrolimus species (Lepidoptera:Lasiocampidae)in China based on allozyme analysis[J]. Acta Entomologica Sinica, 2008, 51(4): 417-423
Authors:NANGONG Zi-Yan  GAO Bao-Jia  LIU Jun-Xia  YANG Jun
Abstract:[Aim] Using polyacrylamide gel electrophoresis to find out the genetic diversity among and within nine geographic populations of four Dendrolimus species in China: Dendrolimus punctatus punctatus Walker, D. punctatus spectabilis Butler, D. superans Butler, D. haui Lajonquiere and D. kikuchii Matsumura. [Methods] Six allozymes, including lactate dehydrogenase (LDH), malate dehydrogenase (MDH), malic enzyme (ME), alcohol dehydrogenase (ADH), formic acid dehydrogenase (FDH) and glutamate dehydrogenase (GDH), were analyzed. [Results] Ten loci and seventeen alleles were recorded with four loci polymorphic, P=40.0%. The average number of alleles per locus A was 1.700, the expected heterozygosity He 0.151, and the mean genetic distance 0.001-0.285. Among six populations of D. punctatus Walker, the coefficient of gene differentiation Fst was 0.265, and the number of migrants per generation Nm 0.692. Genetic relationships among populations and species were assessed by standard genetic distance, and it was found that D. punctatus spectabilis Butler and D. superans Butler had a closer relationship, while D. superans Butler and D. haui Lajonquiere had the farthest relationship. [Conclusions] According to the heredity parameters, gene flow was low and genetic drift was one of primary factors that led to the genetic differentiation among six geographic populations of D. punctatus Walker. Genetic distances were correlated with geographical distances among populations.
Keywords:Dendrolimus  geographical populations  genetic diversity  allozyme  China
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《昆虫学报》浏览原始摘要信息
点击此处可从《昆虫学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号