Ethyl methanesulphonate induced changes in the differentiation, structure and functions of spermatozoa of the house rat,Rattus rattus L. |
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Authors: | Rajinder Kaur V R Parshad |
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Institution: | (1) Department of Zoology, Punjab Agricultural University, 141 004 Ludhiana, India |
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Abstract: | Intraperitoneal administration of 500 mg/kg and 625 mg/kg doses of the germ cell mutagen, ethyl methanesulphonate (EMS) in
5 consecutive days to the house rat,Rattus rattus
caused a dose-dependent reduction in its body weight, cauda epididymides weight, concentration, motility and percentage of
live spermatozoa with simultaneous increase in the percentage of their abnormal forms. Compared to 0·65% spermatozoa with
abnormal heads in the cauda epididymidis of untreated control rats, 24·86% and 65·72% such spermatozoa were observed in rats
on day 14 post treatment with 500 mg/kg and 625 mg/kg doses of EMS respectively. On day 28 post treatment corresponding values
for abnormal spermatozoa were 16·21% and 14·32%. Similarly, spermatozoa with abnormal flagella increased from 0.78% in control
rats to 9·25% and 5·75% on day 14 post treatment of 500 and 625 mg/kg doses of EMS respectively and declined to 2·91% and
2·40% on day 28 post treatment. Abnormality in the sperm head was mainly due to acrosomelessness and in the flagellum due
to bending at proximal region. However, the main effect of EMS was the development of spermatozoa without or deformed acrosomes
which may impair the fertility of rats. Analysis of various stages of differentiation of spermatozoa inthe testis revealed
that population of preleptotene and pachytene spermatocytes and of round spermatids showed a gradual decline which became
significantly less than controls on day 28 of EMS treatment. Occurrence of abnormal heads of testicular spermatids indicated
that the sperm head abnormalities originated in the testis during late spermiogenesis. |
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Keywords: | Acrosomelessness ethyl methanesulphonate sperm abnormalities spermiogenesis |
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