A rapid and simple method for plasmid copy number comparison |
| |
Authors: | Orie Asaka Takashi Ano Makoto Shoda |
| |
Institution: | (1) Research Laboratory of Resources Utilization, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 227 Yokohama, Japan |
| |
Abstract: | Summary A rapid, simple, and sensitive method for plasmid copy number comparison was developed. The extracted plasmids from the same
amount of cells were subjected to agarose gel electrophoresis and the gels photographed. The photographs were processed by
a Macintosh image analyser to enumerate the densities of plasmid bands. As a size reference, λ-DNA digested with a restriction
enzyme was used. The densities divided by size of plasmids (base pair) would represent relative values of their copy numbers. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|