Development of an immunoenzyme method for detection and quantitative determination of microcystins |
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| Authors: | O S Morenkov V V Vrublevskaya N V Kochkina A L Kovtun |
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| Institution: | 1. Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia
2. Scientific Center “Signal”, ul. Bol’shaya Olen’ya 8, Moscow, 107014, Russia
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| Abstract: | An indirect competitive immunoenzyme method for the quantitative estimation of microcystins (MCs) in water (MC-ELISA) using prepared MC-specific polyclonal antibodies was developed. The threshold concentration of the most widespread and highly toxic MC-LR, which was reliably detected using MC-ELISA, was 0.05 ± 0.01 ng/mL; the 50% inhibition concentration was 0.41 ± 0.05 ng/mL; and the concentration range for the quantitative estimation of MC-LR was 0.1–5.0 ng/mL. The MC-ELISA made it possible to detect MC-LR in water at concentrations 10–20 times lower than the World Health Organization guideline level for drinking water and 100–200 times lower than the allowable MC concentrations in water bodies. A group of cross-reacting MCs and nodularin was detected using MC-ELISA. This method can be applied for monitoring MC concentrations in water bodies and drinking water. |
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