SOI-nanowire biosensor for detection of D-NFATc1 protein |
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| Authors: | K A Malsagova Yu D Ivanov T O Pleshakova A F Kozlov N V Krohin A L Kaysheva I D Shumov V P Popov O V Naumova B I Fomin D A Nasimov |
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| Institution: | 1. Orekhovich Institute of Biomedical Chemistry of Russian Academy of Medical Sciences, ul. Pogodinskaya 10, Moscow, 119121, Russia
2. Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, pr. Lavrentieva 13, Novosibirsk, 630090, Russia
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| Abstract: | The nanowire (NW) detection is one of the fast-acting and high-sensitive methods, which can recognize potentially relevant protein molecules. A NW-biosensor based on the silicon-on-insulator (SOI)-structures has been used for biospecific label-free real time detection of the NFATc1 (D-NFATc1) oncomarker. For this purpose, SOI-nanowires (NWs) were modified with aptamers against NFATc1 used as molecular probes. It was shown that using this biosensor it is possible to reach sensitivity of 10?15 M. This sensitivity was comparable to that of the NW-biosensor with immobilized antibodies used as macromolecular probes. The results demonstrate that approaches used in this study are promising for development of sensor elements for high-sensitive diagnostics of diseases. |
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