Stella-Cre mice are highly efficient Cre deleters |
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Authors: | Liu Hui Wang Wei Chew Su-Kit Lee Song-Choon Li Juan Vassiliou George S Green Tony Futreal P Andrew Bradley Allan Zhang Shujun Liu Pentao |
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Affiliation: | College of Animal Science and Technology, Huazhong Agriculture University, Wuhan, China. |
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Abstract: | Cre-loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella-Cre, where Cre expression cassette was targeted to the 3' UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre-loxP recombination efficiency in Stella-Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre-loxP recombination efficiency in the embryos and in the germline. Thus, Stella-Cre mice serve as a very efficient deleter line. |
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Keywords: | Stella Cre‐loxP recombination knock‐in |
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