温度对南疆沙蜥和密点麻蜥消化酶活性的影响

为探究不同温度对南疆沙蜥Phrynocephalus forsythii和密点麻蜥Eremias multiocellata消化酶活性的影响,本研究测定了不同温度下南疆沙蜥和密点麻蜥的肝脏、胃及十二指肠的消化酶——淀粉酶(碘-淀粉比色法)、脂肪酶(比浊法)及胰蛋白酶(紫外线吸收法)的活性。结果显示,南疆沙蜥和密点麻蜥消化酶活性在4～45℃随着温度升高呈现先上升、后下降的趋势;40℃驯化的南疆沙蜥、35℃驯化的密点麻蜥肝脏、胃及十二指肠的淀粉酶、脂肪酶及胰蛋白酶活性最高。南疆沙蜥和密点麻蜥消化酶活性具有温度依赖性,这与机体在不同温度下的生理机能是密切相关的。同时,2种蜥蜴的消化酶活性最大时的驯化温度明显不同,这与它们偏好的环境温度密切相关。

Effect of Temperature on Digestive Enzyme Activities in Phrynocephalus forsythii and Eremias multiocellata

To investigate the effect of different temperatures on digestive enzyme activities in Phrynocephalus forsythii and Eremias multiocellata, the amylase activity (iodine-starch colorimetry), lipase activity (turbidimetry) and trypsin activity (ultraviolet absorption method)in the liver, stomach and duodenum samples of P. forsythii and E. multiocellata under different temperatures were assayed, respectively. The results showed that in the temperature range of 4-45 ℃, the digestive enzyme activities increased first and then decreased with temperature increasing. At 40 ℃, the activities of amylase, lipase and trypsin in the liver, stomach and duodenum of P. forsythii reached the highest level, while the temperature for the highest enzyme activities of E. multiocellata was 35 ℃. These results suggested that the digestive enzyme activities of P. forsythii and E. multiocellata were related with temperature and this relationship also involved the physical states of lizards at different temperatures. Moreover, these findings revealed that the acclimation temperatures of the 2 lizards were significantly different when digestive enzyme activities were maximal, and these were accordant to the preferred temperature of the 2 lizards.