17-beta-estradiol activates maxi-K channels through a non-genomic pathway in human breast cancer cells |
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Authors: | Coiret Guyllaume Matifat Fabrice Hague Frédéric Ouadid-Ahidouch Halima |
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Institution: | Laboratoire de Physiologie Cellulaire et Moléculaire, EA 2086, Université de Picardie Jules Verne, Faculté des Sciences, 33, Rue Saint-Leu 80039 Amiens, France. |
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Abstract: | We have investigated the acute effects of 17-beta-estradiol (E2) on K+ channels in MCF-7 breast epithelial cancer cells. E2 induced a rapid and irreversible augmentation of the K+ current for all membrane potentials superior to -25 mV. The effect of E2 was sensitive to Iberiotoxin, Charybdotoxin and TEA and can be elicited in the presence of the anti-estrogen ICI 182780 or be mimicked by the membrane impermeant form E2/BSA. Furthermore, E2/BSA was able to stimulate cell proliferation in a maxi-K inhibitors-sensitive manner. Thus, these results permit us to identify the maxi-K channel as the molecular target of E2 that regulates cell proliferation independently of the estrogen receptor. |
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Keywords: | Maxi-K Estrogens Non-genomic effect Breast cancer Cell proliferation |
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