Identification of Nsp100 as elongation factor 2 (EF-2) |
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| Authors: | S Koizumi A Ryazanov T Hama H C Chen G Guroff |
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| Institution: | Section on Growth Factors, National Institute of Child Health and Human Development, Bethesda, MD 20892. |
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| Abstract: | The nerve growth factor-sensitive phosphoprotein from PC12 cells, previously designated Nsp100, has been shown to be elongation factor 2 (EF-2). The criteria used for this identification include: (i) similarity of N-terminal sequence; (ii) phosphorylation by the same kinase; (iii) ADP-ribosylation mediated by diphtheria toxin; (iv) comparable function in cell-free protein synthesis. According to these criteria, Nsp100 and EF-2 are identical and the kinase that phosphorylates Nsp100 in PC12 cells is calcium/calmodulin kinase III. |
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