Strain-Dependent Differences in the Efficiency of Transgenic Mouse Production |
| |
Authors: | Auerbach Anna B Norinsky Rada Ho Weilan Losos Kasia Guo Qiuxia Chatterjee Samprit Joyner Alexandra L |
| |
Institution: | (1) Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA;(2) Stern School of Business, NYU, Management Education Center, 44 West 4th Street, New York, NY 10012, USA;(3) Howard Hughes Medical Institute and Department of Cell Biology, NYU School of Medicine, 540 First Avenue, New York, NY 10016, USA |
| |
Abstract: | Transgenic mouse production via pronuclear microinjection is a complex process consisting of a number of sequential steps. Many different factors contribute to the effectiveness of each step and thus influence the overall efficiency of transgenic mouse production. The response of egg donor females to superovulation, the fertilization rate, egg survival after injection, ability of manipulated embryos to implant and develop to term, and concentration and purity of the injected DNA all contribute to transgenic production efficiency. We evaluated and compared the efficiency of transgenic mouse production using four different egg donor mouse strains: B6D2/F1 hybrids, Swiss Webster (SW) outbred, and inbred FVB/N and C57BL/6. The data included experiments involving 350 DNA transgene constructs performed by a high capacity core transgenic mouse facility. Significant influences of particular genetic backgrounds on the efficiency of different steps of the production process were found. Except for egg production, FVB/N mice consistently produced the highest efficiency of transgenic mouse production at each step of the process. B6D2/F2 hybrid eggs are also quite efficient, but lyze more frequently than FVB/N eggs after DNA microinjection. SW eggs on the other hand block at the 1-cell stage more often than eggs from the other strains. Finally, using C57BL/6 eggs the main limiting factor is that the fetuses derived from injected eggs do not develop to term as often as the other strains. Based on our studies, the procedure for transgenic mouse production can be modified for each egg donor strain in order to overcome any deficiencies, and thus to increase the overall efficiency of transgenic mouse production. |
| |
Keywords: | egg donor mouse genetic backgrounds mouse strains pronuclear microinjection transgenesis transgenic mouse production |
本文献已被 PubMed SpringerLink 等数据库收录! |
|