The effect of 7-oxa-13 prostynoic acid on the mechanism of action of bradykinin in human synovial fibroblasts |
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Authors: | John V. Fahey David S. Newcombe |
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Affiliation: | Rheumatology Unit, Department of Medicine University of Vermont, Burlington, Vermont 05401, USA |
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Abstract: | Bradykinin, a potent inflammatory mediator, induces an increment in intracellular cyclic AMP concentrations of human synovial fibroblasts and evokes the synthesis and release of 3H-arachidonic acid and 3H-E prostaglandins from these cells pre-labeled in their phospholipids. Fetal calf serum in the media also stimulates the synthesis and release of these labeled lipids from pre-labeled human synovial fibroblasts and potentiates the bradykinin-induced cyclic AMP response. The PGE1 analogue, 7-oxa-13 prostynoic acid, completely abrogates both the bradkinin-induced cyclic AMP response and the bradykinin- and fetal calf serum-evoked release of labeled E-prostaglandins from pre-labeled cells. In serum-free media, the prostaglandin antagonist stimulated the release of 3H-arachidonic acid from pre-labeled human synovial fibroblasts and did not inhibit the bradykinin-induced release of this lipid. |
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Keywords: | Requests for reprints should be addressed to David S. Newcombe M.D. at The Johns Hopkins University School of Hygiene and Public Health Department of Environmental Health Sciences 615 North Wolfe Street Baltimore Maryland 21205 USA. |
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