Influence of plant growth hormones on the growth of Mucor rouxii and chitosan production

Supplementation of molasses-salt medium with plant growth hormones, viz., indoleacetic acid, indolebutyric acid, kinetin and gibberellic acid, increased chitosan production by Mucor rouxii as well as its growth at different optimum concentrations. The increase in yield of chitosan was found to range from 34% to 69% and mycelial growth from 12% to 17.4%. Gibberellic acid was the most potent in this respect. Sixty-nine percent more chitosan over the control could be obtained from 1l of the medium supplemented with 3mg gibberellic acid. Degree of acetylation of chitosan ( approximately 13%) was not changed due to addition of hormone in the medium but weight average molecular weight of chitosan increased by more than 50%. Thus, the plant growth hormones add a value to chitosan by increasing its molecular weight.