不同浓度的La^3＋和Cu^2＋对银杏固体培养和液体培养组织中黄酮产量的影响

以不同浓度的La^3 及Cu^2 分别加入到银杏愈伤组织的固体培养基和液体培养基中，发现La^3 浓度与细胞中黄酮含量呈负相关性；而随着Cu^2 浓度的提高，细胞中黄酮含量也提高；加入前体物(0．05g／L的苯丙氨酸和0．2g／L的乙酸钠)后，这种作用会更显著，黄酮含量比对照增加1倍到2倍．而随着Cu^2 浓度的升高，细胞生长会受到轻微的抑制，加入前体后，这种抑制作用会被缓解．Cu^2 可以认为是一种良好的非生物诱导子。

The effect of different concentrations of La3+ and Cu2+ on flavonoid production in solid and liquid culture of Ginkgo biloba

The different concentrations of La3+ and Cu2+ are respectively added to solid media and liquid media to culture the callus of Ginkgo biloba L.. There are obvious results that concentrations of La3+ have the adverse correlation with flavonoid production; and with the concentrations of Cu2+ increasing, the flavonoid content of the examples enhances; and the action is more significant after adding precursors(0.05 g/L phenylalanine and 0.2 g/L sodium acetate). The result shows flavonoid production will be increased from one time to two times, compared with the control. And the cell growth can be slightly inhibited by Cu2+, and the inhibition action can be relieved by precursors. Cu2+ can be regarded as a proper abiotic elicitor.