Effects of pH and deuterium oxide on the heat-inactivation temperature of chloroplasts |
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| Authors: | Pavel S. Venediktov Alla A. Krivoshejeva |
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| Affiliation: | (1) Biophysics Department, Faculty of Biology, M.V. Lomonosov State University, Lenin Hills, 117234 Moscow, USSR |
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| Abstract: | The inactivation temperature for Hill activity and for the long-lived delayed fluorescence of isolated Pisum sativum L. chloroplasts was found to depend on pH, the maximal value being in the pH region 5–7. Salts increase the inactivation temperature by 4–7°C. Effects of D2O and some other substances that modify the thermostability of chloroplasts are dependent on pH. It is concluded that thermal denaturation of proteins is the most probable mechanism for heat inactivation of chloroplasts.Abbreviations Hepes 4-(2-hydroxymethyl)-1-piperazineethane sulfonic acid - Tris 2-amino-2-(hydroxymethyl)-1,3-propanediol |
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| Keywords: | Chloroplast (heat inactivation) Fluorescence, delayed Hill reaction Pisum (chloroplast) Synechococcus |
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