Non-destructive RAPD genetic diagnostics of microspore-derivedBrassica embryos |
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| Authors: | Peggy Horn Antoni Rafalski |
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| Institution: | (1) Agricultural Products-Biotechnology, DuPont Co. Experimental Station, P.O. Box 80402, 19880 Wilmington, DE, USA;(2) University of Canberra-Applied Sciences POB1, 2616 Belconnen, ACT, Australia |
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| Abstract: | We demonstrate the application of the RAPD genetic polymorphism assay (Williams et al., 1990, Welsh and McClelland 1990),
to the determination of the genotype of immature, microspore-derived haploid embryos ofBrassica napus. Several hundred assays can be performed on the DNA obtained from a cotyledon fragment, and the remaining embryo can be regenerated.
Thus, the assay can be used for ”prenatal” diagnostics of embryo-regenerated plants, and can facilitate selection of defined
genotypes in plant breeding. A suitable population of embryos could also be used for the construction of RAPD genetic maps. |
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| Keywords: | Genetic mapping RAPD random primers Brassica microspore-derived embryo |
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