Bile acid catabolites accumulated by transposon-induced mutants ofPseudomonas
putida: Production of hydroxylated 1,4-androstadiene-3,17-diones |
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| Authors: | J A Ide R J Park N W Dunn |
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| Institution: | (1) Biotechnology, University of New South Wales, P.O. Box 1, 2033 Kensington, N.S.W., Australia;(2) Meat Research Laboratory, CSIRO Division of Food Research, Cannon Hill, 4170 Queensland |
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| Abstract: | Summary Transposon mutagenesis of a bile acid-utilizingPseudomonas
putida strain generated 5 classes of mutants based on their ability to accumulate steroild catabolites. Bile acids (up to 5%) could be fermented using mutant strains, to the appropriate hydroxy-1,4-androstadiene-3,17-dione type product in yields close to theoretical. |
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