Repeated batch production of epothilone B by immobilized Sorangium cellulosum |
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Authors: | Park Woo Sang Park Su Jeong Han Se Jong Lee Jinwon Kim Dong-Shik Kim Ji-Heung Kim Byung Woo Lee Jeewon Sim Sang Jun |
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Affiliation: | Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea. |
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Abstract: | Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day). |
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