The potential of 1,1'-hexamethylenebis [3-(3,5-dichloro-4-pyridyl)] urea to modify genotoxic actions of chemical mutagens in various test-systems

The main tasks of this investigation were to investigate the potential genotoxic effect of 1,1'-hexamethylenebis 3-(3,5-dichloro-4-pyridyl)] urea and to analyze its capacity to induce adaptive response (AR) against chemical mutagens in various test-systems. Microbiological, cytogenetical and biochemical end-points were used. The sensitivity of test systems can be arranged as followed: human lymphocyte cultures > Chlamydomonas reinhardtii > Hordeum vulgare. It was obtained that HMPU can induce oxidative stress in Chlamydomonas reinhardtii cells (7.5 x 10(-4) mol/l) and at appropriate experimental conditions can trigger an AR against chemical mutagens in Hordeum vulgare (7.5 x 10(-3) mol/l) and human lymphocytes (10(-5), 10(-6) mol/l). The extent of the AR induction was closely connected with the increase of the inter-treatment time between the conditioning concentration of HMPU and the challenge concentration of chemical mutagens.