Effects of molsidomine on retinal ischemia/reperfusion injury in rabbits |
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Authors: | N Polat MA Ozer H Parlakpinar N Vardi Z Aksungur O Ozhan |
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Institution: | 1. Department of Ophthalmology, Inonu University School of Medicine, Malatya;2. Department of Ophthalmology, Giresun University School of Medicine, Giresun;3. Departments of Pharmacology;4. Embryology and Histology;5. Biochemistry, Inonu University School of Medicine, Malatya, Turkey |
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Abstract: | We investigated the effect of molsidomine (MOL) on ischemia/reperfusion (I/R) injury. Rabbits were assigned to four groups: group 1, sham; group 2, I/R; group 3, MOL treatment for 4 days after I/R; group 4, MOL treatment for 1 day before I/R and 3 days after I/R. Retinal I/R was produced by elevating the intraocular pressure to 150 mm Hg for 60 min. Seven days after I/R, the eyes were enucleated. Retinal changes were examined using histochemistry. The levels of malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) also were measured. We found a significant increase in the thickness of the outer nuclear layer of group 3 compared to the other groups. In groups 3 and 4, caspase-3 stained cells in the ganglion cell layer were decreased compared to group 2. We found a significant increase in caspase-3 stained cells in the inner nuclear layer (INL) of group 2 compared to the other groups. We found a significant increase in caspase-3 stained cells in group 3 compared to group 4 in the INL. The MDA level in group 2 was significantly higher than group 1 and MOL significantly decreased MDA levels in groups 3 and 4. We found that MOL protected the retina from I/R injury by enhancing antioxidative effects and inhibiting apoptosis of retinal cells. |
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Keywords: | apoptosis ischemia/reperfusion molsidomine oxidative stress rabbit retina |
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