EB病毒感染及XRCC1、IL-10基因多态性与甲状腺癌的关联性分析

摘要 目的：探究Epstein-Barr病毒（EB病毒）感染及X射线交错互补修复因子1（XRCC1）、白介素-10（IL-10）基因多态性与甲状腺癌的关联性。方法：选取2020年1月~2022年12月132例甲状腺癌患者为研究组以及同期132例甲状腺良性腺瘤患者为对照组，采用原位杂交技术检测肿瘤标本EB病毒感染情况，聚合酶链反应-限制性内切酶片段长度多态性法检测XRCC1-399G/A位点、IL-10-592C/A位点基因多态性。结果：研究组EB病毒感染阳性率55.3%，高于对照组的33.3%（P<0.05）。研究组XRCC1-399G/A位点GA、AA基因型及A等位基因频率均高于对照组（P<0.05）；两组IL-10-592C/A各基因型频率比较差异无统计学意义，但研究组A等位基因频率高于对照组（P<0.05）。EB病毒感染阳性者较阴性者甲状腺癌风险增加3.337倍（95%CI：1.272~8.752），携带XRCC1-399位点（GA+AA）型者较GG型风险增加2.438倍（95%CI：1.223~4.859），携带IL-10-592位点（CA+AA）型者较CC型未增加甲状腺癌风险。不同病理类型甲状腺癌患者EB病毒感染情况及XRCC1-399位点、IL-10-592位点基因型分布比较差异均无统计学意义。结论：EB病毒感染阳性、XRCC1-399G/A位点突变基因型可能是甲状腺癌发病的易感因素，但二者与甲状腺癌病理类型无明显关系，而IL-10-592C/A基因多态性可能与甲状腺癌无关。

Association of EB Virus Infection and Gene Polymorphisms of XRCC1 and IL-10 with Thyroid Cancer

ABSTRACT Objective: To explore the association between Epstein-Barr virus (EB virus) infection and gene polymorphisms of X-ray cross complementary repair gene-1 (XRCC1) and interleukin-10 (IL-10) and thyroid cancer. Methods: 132 patients with thyroid cancer from January 2020 to December 2022 were selected as study group and 132 patients with benign thyroid adenoma during the same period were enrolled as control group. In situ hybridization was used to detect EB virus infection in tumor specimens. The gene polymorphisms of XRCC1-399G/A locus and IL-10-592C/A locus were detected by polymerase chain reaction-restriction fragment length polymorphism. Results: The positive rate of EB virus infection in study group was 55.3%, which was higher than 33.3% in control group (P<0.05). The frequencies of GA and AA genotypes and A allele at XRCC1-399G/A locus in study group were higher than those in control group (P<0.05). There were no statistical differences in the frequencies of IL-10-592C/A genotypes between the two groups, but the A allele frequency in study group was higher than that in control group (P<0.05). The risk of thyroid cancer was 3.337 times (95%CI: 1.272-8.752) higher in patients with positive EB virus infection than patients with negative EB virus infection. The risk of thyroid cancer was 2.438 times (95%CI: 1.223-4.859) higher in patients carrying (GA+AA) at XRCC1-399 locus than patients with GG genotype. The risk of thyroid cancer did not increase in patients with (CA+AA) genotype at IL-10-592 locus compared with patients with CC genotype. There were no statistically significant differences in EB virus infection and genotype distribution of XRCC1-399 locus and IL-10-592 locus among patients with different pathological types of thyroid cancer. Conclusion: Positive EB virus and mutant genotypes at XRCC1-399G/A may be predisposing factors to thyroid cancer, but there is no significant relationship between them and pathological types of thyroid cancer, and IL-10-592C/A gene polymorphism may not be associated with thyroid cancer.