High-performance chromatographic assay for the sulphoxide metabolite of 2′-deoxy-3′-thiacytidine in human urine |
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| Authors: | R.S. Plumb R.D.M. Gray A.J. Harker S. Taylor |
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| Affiliation: | aBioanalysis and Drug Metabolism Department, GlaxoWellcome Research and Development, Greenford Road, Greenfjord, Middlesex, UB6 OHE, UK;bDepartment of Pharmacology, University of Leeds, Leeds, LS2 9JT, UK |
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| Abstract: | A high-performance liquid chromatographic method is described for the determination in human urine of GI138870X, the sulphoxide metabolite of a novel dideoxynucleoside analogue, 2′-deoxy-3′-thiacytidine (lamivudine). GI138870X was extracted from human urine using Empore SDB RPS solid-phase extraction disks prior to reversed-phase chromatography with UV detection. The method has shown to be valid over the concentration range 0.5–100 μg/ml using a 0.5-ml sample volume. |
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| Keywords: | Lamivudine 2′ -Deoxy-3′ -thiacytidine |
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