Application of the comprehensive set of heterozygous yeast deletion mutants to elucidate the molecular basis of cellular chromium toxicity |
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| Authors: | Sara Holland Emma Lodwig Theodora Sideri Tom Reader Ian Clarke Konstantinos Gkargkas David C Hoyle Daniela Delneri Stephen G Oliver Simon V Avery |
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| Affiliation: | (1) School of Biology, Institute of Genetics, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK;(2) North West Institute for Bio-Health Informatics, The University of Manchester, ISBE, School of Medicine, Oxford Road, Manchester, M13 9PT, UK;(3) Department of Biochemistry, University of Cambridge, Sanger Building, Tennis Court Road, Cambridge, CB2 1GA, UK;(4) Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT, UK |
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| Abstract: | Background The serious biological consequences of metal toxicity are well documented, but the key modes of action of most metals are unknown. To help unravel molecular mechanisms underlying the action of chromium, a metal of major toxicological importance, we grew over 6,000 heterozygous yeast mutants in competition in the presence of chromium. Microarray-based screens of these heterozygotes are truly genome-wide as they include both essential and non-essential genes. |
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