| 一种新型肿瘤细胞抑制蛋白的分离纯化 |
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| 引用本文： | 陶海南,刘辉,刘全俊,陈贵泉,欧阳献,舒志群.一种新型肿瘤细胞抑制蛋白的分离纯化[J].中国生物化学与分子生物学报,1997,13(2):216-220. |
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| 作者姓名： | 陶海南 刘辉 刘全俊 陈贵泉 欧阳献 舒志群 |
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| 作者单位： | 南昌大学生物科学工程系!南昌，330047(陶海南，刘全俊，陈贵泉，欧阳献，舒志群)，江西省儿童医院!南昌，330006(刘辉) |
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| 基金项目： | 江西省一级重点资助!8903103 |
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| 摘 要： | 介绍了从健康牛骨髓中分离到一种具有抑制肿瘤活性的糖蛋白，并对该糖蛋白进行了纯化，获得了电泳纯和层析纯的纯品．测得该糖蛋白N末端为两氨酸残基．用SDS-PAGE法测得该溏蛋白的表现分子量为65kD．氨基酸组成分析显示其为一种富含丝氨酸的蛋白质．生物活性测定表明，该糖蛋白纯品对小白鼠白血病P388细胞和人慢粒白血病HL-60细胞株的增殖均有抑制作用．有关该糖蛋白的糖含量的定量测定及蛋白质一级结构分析正在进一步的研究中．
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| 关 键 词： | 糖蛋白 肿瘤 纯化 SDS-PAGE |
| 收稿时间： | 1997-04-20 |
Studies on A New Tumor Cell Growth Inhibiting Protein |
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| Tao Hai-Nan,Liu Hui,Liu Quan-Jun,Chen Gui-Quan,Ouyang-Xian,Shu Zhi-Qun.Studies on A New Tumor Cell Growth Inhibiting Protein[J].Chinese Journal of Biochemistry and Molecular Biology,1997,13(2):216-220. |
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| Authors: | Tao Hai-Nan Liu Hui Liu Quan-Jun Chen Gui-Quan Ouyang-Xian Shu Zhi-Qun |
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| Institution: | (Department of Biological Science and Biotechnology, Nanchang University, Nanchang 330047) (Pediatrics Hospital of Jiangxi Province,Nachang 330006 |
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| Abstract: | A new tumor cell growth inhibiting glycoproten was isolated from bovine narrow and purified with RP-HPLC. The molecular weight of the protein is about 65 kD estimated by SDS-PAGE and sucrose denSity gradient zonal. centrifugation methods. The amino acids composition analysis of the protein was performed with HPLC. The protein consists of 16 kinds of amino acid residues especially rich in Serine residue. It possesses obvious functions in repressing the growth of P388 and HL-60 tumor cells. |
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| Keywords: | Tumor Glycoprotein Purification SDS-PAGE |
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