Alkaline phosphatase production during sporulation ofBacillus cereus

Cell-bound alkaline phosphatase ofBacillus cereus was produced during vegetative growth and sporulation in a complex medium. Addition of glucose repressed the sporulation process and the amount of enzyme synthesized increased. The time course of alkaline phosphatase production is very similar in both sporulating and non-sporulating cells. Irrespective of sporulation, alkaline phosphatase level shows a peak of activity in the exponential phase, and another in the stationary phase of growth. This preliminary data indicates differences betweenB. cereus, andB. subtilis in alkaline phosphatase characteristics.