Isozyme expression in F1 hybrids between carp and goldfish |
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Authors: | Roy G Danzmann Norman E Down |
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Institution: | (1) Department of Zoology, College of Biological Science, University of Guelph, N1G 2W1 Guelph, Ontario, Canada |
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Abstract: | Interspecific genetic differences in malate dehydrogenase (MDH), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and esterase (EST) isozymes in carp (Cyprinus carpio) and goldfish (Carassius auratus) were used to examine the allelic expressions in the hybrid between these species. A unique liver SOD and muscle LDH phenotype unambiguously identifies all presumed hybrid individuals. There was no evidence of F2 or backcross phenotypes in hybrid individuals. Liver MDH and EST phenotypes in hybrids show a preferential expression of goldfish isozymes. Variation in the levels of carp liver MDH isozymes may result from the polymorphism of a regulatory mutation affecting isozyme expression, leading to gene silencing after duplication.This work was supported through NSERC (Canada) grants to James P. Bogart and John F. Leatherland. |
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Keywords: | carp goldfish hybrids electrophoresis gene silencing |
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