Cell adaptation to solvent, substrate and product: a successful strategy to overcome product inhibition in a bioconversion system |
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Authors: | Carla C C R de Carvalho Alessandro Poretti M Manuela R da Fonseca |
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Institution: | (1) Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal |
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Abstract: | Carvone has previously been found to highly inhibit its own production at concentrations above 50 mM during conversion of a diastereomeric mixture of (−)-carveol by whole cells of Rhodococcus erythropolis. Adaptation of the cells to the presence of increasing concentrations of carveol and carvone in n-dodecane prior to biotransformation proved successful in overcoming carvone inhibition. By adapting R. erythropolis cells for 197 h, an 8.3-fold increase in carvone production rate compared to non-adapted cells was achieved in an air-driven column reactor. After an incubation period of 268 h, a final carvone concentration of 1.03 M could be attained, together with high productivity 0.19 mg carvone h−1 (ml organic phase)−1] and high yield (0.96 g carvone g carveol−1). |
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