Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media |
| |
Authors: | Zdeněk Lukáš Karel Dvořák Zdeňka Juránková |
| |
Affiliation: | (1) 2nd Department of Pathology of the Medical Faculty UJEP, Children's Hospital, Brno, SSR |
| |
Abstract: | Summary The presence of 1% agar in the fixation and substrate solutions for the histochemical demonstration of thiamine pyrophosphatase (4.4 mM TPP; 3.6 mM Pb2+; 0.025 Tris-maleate buffer, pH 7.2) clearly facilitates the localization of the enzyme in Golgi apparatus in cold microtome sections prepared from unfixed specimens. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |