Bioremediation of polyaromatic hydrocarbon contaminated soils by native microflora and bioaugmentation with Sphingobium chlorophenolicum strain C3R: A feasibility study in solid- and slurry-phase microcosms |
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Authors: | Milena ColomboLucia Cavalca Silvana BernasconiVincenza Andreoni |
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Affiliation: | a Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Facoltà di Agraria, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy b Dipartimento di Chimica Organica e Industriale, Università degli Studi di Milano, Via Venezian 21, 20133 Milano, Italy |
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Abstract: | The aim of the research was to verify if a Sphingobium chlorophenolicum strain C3R was effective in the degradation of phenanthrene (Ph) in agricultural soil co-contaminated by metals and mixtures of PAHs. The presence of PAHs in mixtures produced interactive effects which could either increase or decrease the utilization rate of Ph by C3R and by the native bacterial microflora. Bioaugmentation significantly improved the biodegradation rate of Ph in the presence of both cadmium and arsenic and PAH mixtures. The augmented C3R strain persisted in inoculated microcosms as monitored by the DGGE analysis and outcompeted some indigenous bacteria. The potential role of the soil bacteria in PAH degradation could be envisaged. The results indicate the applicability of S. chlorophenolicum C3R toward in situ bioremediation of sites contaminated with phenanthrene alone or co-contaminated with low molecular weight PAHs and with cadmium and arsenate. |
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Keywords: | PAHs Metals Bioaugmentation DGGE |
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