Removal of malachite green from aqueous solution by immobilized Pseudomonas sp. DY1 with Aspergillus oryzae |
| |
Authors: | Yuyi YangHua Hu Guan WangZeli Li Bing WangXiaoming Jia Yuhua Zhao |
| |
Affiliation: | College of Life Sciences, Zhejiang University, Yuhangtang Road, Hangzhou 310058, China |
| |
Abstract: | Triphenylmethane dyes are considered to be one of the most recalcitrant pollutants in the environment. Malachite Green (MG) was successfully removed from aqueous solution by Pseudomonas sp. DY1 immobilization with Aspergillus oryzae. Inhibition test in the presence of sodium azide and nystatin indicated that A. oryzae was a natural immobilization reagent, and removal of MG by the immobilized cell pellets was attributed to the biodegradation by Pseudomonas sp. DY1. Optimum conditions of immobilization for maximum biodegradation were obtained using Taguchi design at 37 °C, inoculation size of Pseudomonas sp. DY1 (dry cell mass) 0.01 g, of A. oryzae (spore number) 1.0 × 109, initial pH 6.5. Decolorization and biodegradation of MG by immobilized pellets under optimum conditions were 99.5% and 93.3%, respectively. Immobilized pellets exhibited more than 96% decolorization after 16 days in batch condition, indicating it had stable and high biodegradation capabilities when immobilized for long-term operation. |
| |
Keywords: | Pseudomonas sp. DY1 Aspergillus oryzae Immobilization Malachite green Taguchi design |
本文献已被 ScienceDirect 等数据库收录! |
|