Characterization of feather-degrading bacteria from Brazilian soils |
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Authors: | Evelise BachFabiana S. Cannavan,Fá bio R.S. DuarteJaslin A.S. Taffarel,Siu Mui TsaiAdriano Brandelli |
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Affiliation: | a Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência e Tecnologia de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazilb Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil |
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Abstract: | Studies on keratinolytic microorganisms have been mainly related to their biotechnological applications and association with animal pathologies. However, these organisms have an ecological relevance to recycling keratinous residues in nature. This work aimed to select and identify new culturable feather-degrading bacteria isolated from soils of Brazilian Amazon forest and Atlantic forest. Bacteria that were isolated from temperate soils and bacteria from Amazonian basin soil were tested for their capability to grow on feather meal agar (FMA). Proteolytic bacteria were tested for feather degradation and were further identified according to their morphological and biochemical characteristics. Also, molecular identification based on 16S rDNA gene sequencing was carried out. A total of 24 proteolytic and 20 feather-degrading isolates were selected; Most of the isolates were from the Bacillus genus (division Firmicutes), but one Aeromonas, two Serratia (??-Proteobacteria), and one Chryseobacterium (Cytophaga-Flavobacterium group). |
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Keywords: | Isolation Brazilian soils Keratinolytic proteases Feather degradation Bacillus |
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