Improved enzyme production by co-cultivation of Aspergillus niger and Aspergillus oryzae and with other fungi |
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| Authors: | H.L. Hu,J. van den BrinkB.S. Gruben,H.A.B. Wö sten,J.-D. GuR.P. de Vries |
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| Affiliation: | a Microbiology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlandsb School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, Hong Kong SAR, Chinac Ministry of Agriculture Key Laboratory of Subtropical Agro-biological Disaster and Management, Fujian Agriculture and Forestry University, Fuzhou 350002, Chinad CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlandse Kluyver Centre for Industrial Fermentation, Utrecht University, Utrecht, The Netherlands |
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| Abstract: | Aspergillus niger and Aspergillus oryzae were co-cultivated with each other and with Magnaporthe grisea or Phanerochaete chrysosporium, respectively. Enzyme assays for plant polysaccharide and lignin-degrading enzymes showed that co-cultivation can improve extracellular enzyme production. Highest ??-glucosidase, ??-cellobiohydrolase, ??-galactosidase, and laccase activities were found for A. oryzae in combination with other fungi, in particular with P. chrysosporium. Highest ??-xylosidase activity was obtained when A. niger was co-cultivated with P. chrysosporium. SDS-PAGE protein profiles demonstrated that A. niger and A. oryzae contributed most to the overall enzyme activities found in the culture medium of the mixed cultivations. These data demonstrate that co-cultivation of two major industrial fungi, A. niger and A. oryzae, results in improved production of biotechnologically relevant enzymes. |
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| Keywords: | Aspergillus Co-cultivation Polysaccharide degradation Lignin degradation Enzyme production |
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