Efficient identification and regional positioning of YAC and cosmid clones to human Chromosome 21 by radiation fusion hybrids |
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| Authors: | J. Kumlien T. Labella G. Zehetner R. Vatcheva D. Nizetic H. Lehrach |
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| Affiliation: | (1) Department of Genome Analysis, Imperial Cancer Research Fund, 44, Lincoln's Inn Fields, WC2A 3PX, London, UK;(2) Institute of Protein Biochemistry and Enzymology (CNR), Naples, Italy |
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| Abstract: | The use of integrated mapping strategies involving bacterial, yeast, and rodent cells as hosts simplifies the construction of maps, which combine long-range order, high resolution, and easy access to the cloned DNA. Radiation-fusion hybrids offer a specially powerful long-range mapping system for human chromosomes. We describe here techniques for establishing a radiation-fusion hybrid map of Chromsome (Chr) 21 q and its integration with local information on YAC and cosmid positions. |
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