The chromate resistance phenotype of some yeast mutants correlates with a lower level of Cr(V)-species generated in the extra-cellular medium |
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Authors: | Helena Ksheminska Taras Honchar Yuriy Usatenko Galina Gayda Mykhailo Gonchar |
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Institution: | 1.Department of Analytical Biotechnology,Institute of Cell Biology, NAS of Ukraine,Lviv,Ukraine;2.Faculty of Physics,Ivan Franko National University of Lviv,Lviv,Ukraine;3.Branch Campus of the Faculty of Biotechnology,Rzeszów University,Kolbuszowa,Poland |
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Abstract: | The paper describes the selection of chromate-resistant mutants of the yeast Pichia guilliermondii with a higher chromate-reducing activity and reports the EPR-study of Cr(V)-generation in the extra-cellular medium during
the reduction of chromate by the yeast culture. It is shown that the reduction of chromate to Cr(III) species runs through
the extra-cellular generation of Cr(V)-intermediate(s), thus supporting the assumption about the existence of an extra-cellular
pathway of Cr(VI)-reduction. Furthermore, it is demonstrated that the chromate-resistance phenotype of tested mutants correlates
with a lower stationary level of Cr(V)-species in the medium. It is thus suggested that isolated mutants can be used as sources
of Cr(III)-biocomplexes due to their ability to effectively reduce chromate to Cr(III)-chelates with potential pharmacological
applications. |
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