Evolution of CXCR4-using human immunodeficiency virus type 1 SF162 is associated with two unique envelope mutations |
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Authors: | Kiselyeva Yana Nedellec Rebecca Ramos Alejandra Pastore Cristina Margolis Leonid B Mosier Donald E |
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Affiliation: | Laboratory of Molecular and Cellular Biophysics, National Institute of Child Health and Human Development/NIH, 10 Center Drive, Bethesda, MD 20892, USA. |
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Abstract: | CCR5-using human immunodeficiency virus type 1 (HIV-1) isolates typically gain CXCR4 use via multiple mutations in V3 and often V1/V2 regions of envelope, and patterns of mutations are distinct for each isolate. Here, we report that multiple CXCR4-using variants of a parental CCR5-using HIV-1 isolate, SF162, obtained by either target cell selection or CCR5 inhibition have a common mutation pattern characterized by the same two V3 mutations and that these mutations preexisted in some of the SF162 stocks. These results imply that SF162 has a single pathway for acquiring CXCR4 use and that prolonged culture is sufficient to select for R5X4 variants. |
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