Detection and characterization of chitinases and other chitin-modifying enzymes |
| |
Authors: | Michael?B?Howard Nathan?A?Ekborg Ronald?M?Weiner Email author" target="_blank">Steven?W?HutchesonEmail author |
| |
Institution: | (1) Department of Cell Biology and Molecular Genetics, University of Maryland , Microbiology Bldg, College Park, MD 20742, USA;(2) Division of Molecular and Cellular Biosciences, National Science Foundation, 4201 Wilson Blvd, Arlington, VA 22230, USA |
| |
Abstract: | Multiple industrial and medical uses of chitin and its derivatives have been developed in recent years. The demand for enzymes with new or desirable properties continues to grow as additional uses of chitin, chitooligosaccharides, and chitosan become apparent. Microorganisms, the primary degraders of chitin in the environment, are a rich source of valuable chitin-modifying enzymes. This review summarizes many methods that can be used to isolate and characterize chitin-modifying enzymes including chitin depolymerases, chitodextrinases, chitin deacetylases, N-acetylglucosaminidases, chitin-binding proteins, and chitosanases. Chitin analogs, zymography, detection of reducing sugars, genomic library screening, chitooligosaccharide electrophoresis, degenerate PCR primer design, thin layer chromatography, and chitin-binding assays are discussed. |
| |
Keywords: | Chitin Chitinase Chitooligosaccharide Chitosan Chitosanase |
本文献已被 PubMed SpringerLink 等数据库收录! |
|