Influence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor |
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Authors: | K Nakano M Rischke S Sato H Märkl |
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Institution: | Institute of Bioprocess and Biochemical Engineering, Technical University of Hamburg-Harburg, Denickestra?e 15, D-21071 Hamburg, Germany, DE Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan, JP
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Abstract: | High-cell-density cultivations of Escherichia coli K12 in a dialysis reactor with controlled levels of dissolved oxygen were carried out with different carbon sources: glucose
and glycerol. Extremely high cell concentrations of 190 g/l and 180 g/l dry cell weight were obtained in glucose medium and
in glycerol medium respectively. Different behaviour was observed in the formation of acetic acid in these cultivations. In
glucose medium, acetic acid was formed during the earlier phase of cultivation. However, in glycerol medium, acetic acid formation
started later and was particularly rapid at the end of the cultivation. In order to estimate the influence of acetic acid
during these high-cell-density cultivations, the inhibitory effect of acetic acid on cell growth was investigated under different
culture conditions. It was found that the inhibition of cell growth by acetic acid in the fermentor was much less than that
in a shaker culture. On the basis of the results obtained in these investigations of the inhibitory effect of acetic acid,
and the mathematical predictions of cell growth in a dialysis reactor, the influence of acetic acid on high-cell-density cultivation
is discussed.
Received: 20 May 1997 / Received revision: 12 August 1997 / Accepted: 25 August 1997 |
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Keywords: | Escherichia coli High-cell-density cultivation Acetic acid Inhibition Dialysis reactor |
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