Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples |
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| Authors: | Leuko Stefan Legat Andrea Fendrihan Sergiu Stan-Lotter Helga |
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| Affiliation: | Cyanobacteria and Astrobiology Research Laboratory, University of New South Wales, Sydney, Australia. |
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| Abstract: | Extremophilic archaea were stained with the LIVE/DEAD BacLight kit under conditions of high ionic strength and over a pH range of 2.0 to 9.3. The reliability of the kit was tested with haloarchaea following permeabilization of the cells. Microorganisms in hypersaline environmental samples were detectable with the kit, which suggests its potential application to future extraterrestrial halites. |
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