川芎嗪对家兔肺缺血/再灌注损伤时Fas/FasL基因表达的影响

目的:探讨川芎嗪对肺缺血/再灌注损伤(PI/RI)时Fas/FasL基因表达的影响。方法:采用在体兔单肺原位缺血-再灌注模型。实验兔90只,随机均分为假手术对照组(Sham)、肺缺血/再灌注组(I/R)和肺缺血/再灌注加川芎嗪治疗组(LGT)。每组又分为再灌注1h3、h、5 h三个亚组,每个亚组10只,分别于再灌注1 h3、h、5 h三个时点取左肺组织,观察Fas/Fas配体(Fas/FasL)mRNA定位表达、凋亡指数(AI)、肺组织湿、干重比(W/D)、肺损伤组织学定量评价指标(IQA)及光镜、电镜下的组织形态学改变。结果:肺再灌注1 h、3 h、5 h,LGT组Fas/FasLmRNA在肺小动脉内(外)膜、肺小静脉内膜、肺泡上皮及肺支气管上皮弱阳性表达,与I/R组同一时点比较阳性表达明显减弱;AI、W/D和IQA值显著低于I/R组(P<0.01和P<0.05);肺组织形态学异常改变不同程度减轻。结论:川芎嗪可下调肺组织Fas/FasL mRNA的表达而减轻细胞凋亡,对PI/RI发挥积极的防护作用。

Effect of ligustrazine on expression of Fas/FasL in pulmonary injury induced by ischemia/reperfusion in rabbits

Aim: To investigate the effect of ligustrazini(LGT) on expression of Fas/FasL mRNA during pulmonary ischemia/reperfusion injury(PI/RI) in the rabbits.Methods: Single lung ischemia/reperfusion animal model was used in this study.The rabbits were randomly divided into three groups(n=30,in each):sham operated group(Sham),I/R group(I/R) and I/R LGT group(I/R LGT).Changes of several parameters which included apoptotic index(AI),wet to dry ratio of lung tissue weight(W/D) and index of quantitative assessment of histologic lung injury(IQA) were measured at 1h,3h,5h after reperfusion in lung tissue.Meanwhile the location and expression of Fas/FasL mRNA were observed.Lung tissue was prepared for light microscopic and electron microscopic observation at 1 h,3 h,5 h after reperfusion.Results: As compared with group I/R,Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery,alveoli,and bronchiole epithelia in group LGT.The values of AI,W/D and IQA showed significantly lower in group I/R LGT than that in group I/R at 1 h,3 h,5 h after reperfusion in lung tissue(P<0.01 and P<0.05).Meanwhile,abnormal changes of the lung tissue in morphologically were lessen markedly in group I/R LGT.Conclusion: Ligustrazini has notable protective effects on PI/RI in rabbits by inhibiting Fas/FasL mRNA express in lung tissue and decreasing apoptosis.