Gene Expression in Tilapia Following Oral Delivery of Chitosan-Encapsulated Plasmid DNA Incorporated into Fish Feeds |
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Authors: | Erwin A Ramos Jenne Liza V Relucio Celia Aurora T Torres-Villanueva |
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Institution: | (1) National Institute of Molecular Biology and Biotechnology, College of Science, University of the Philippines, Diliman, Quezon City, 1101, Philippines |
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Abstract: | DNA delivery into fish is important for transient gene expression, (e.g., DNA vaccination). Previous studies have generally
focused on intramuscular injection of DNA vaccines into fish. However, this method is obviously impractical and laborious
for injecting large numbers of fishes. This study reports oral delivery of a construct expressing the β-galactosidase reporter
gene into fish by encapsulating the DNA in chitosan and incorporating it into fish feeds. We found that β-galactosidase expression
could be observed in the stomachs, spleens, and gills of fishes fed with flakes containing the chitosan-DNA complex. These
results suggest that DNA vaccines and other constructs can be easily and cheaply delivered into fishes orally by use of carriers
and incorporation into fish feeds. |
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Keywords: | oral gene delivery transient gene expression plasmid DNA |
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