| 慢性阻塞性肺疾病大鼠模型病变特点的研究 |
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| 引用本文： | 张莉,李金田,刘永琦,李娟,张毅. 慢性阻塞性肺疾病大鼠模型病变特点的研究[J]. 四川动物, 2010, 29(2) |
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| 作者姓名： | 张莉 李金田 刘永琦 李娟 张毅 |
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| 作者单位： | 甘肃中医学院,兰州,730000;甘肃中医学院,兰州,730000;甘肃中医学院,兰州,730000;甘肃中医学院,兰州,730000;甘肃中医学院,兰州,730000 |
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| 基金项目： | 2003甘肃省科计攻关项目,2005甘肃省教育厅导师项目 |
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| 摘 要： | 目的 研究慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠模型肺表面活性物质相关蛋白A(Surfactant protein A, SP-A)表达及肺组织超微结构变化特点.方法 运用气管内滴注脂多糖加烟熏28天法建立COPD大鼠模型.观察大鼠一般状态、用免疫组化法检测SP-A表达及透射电镜观察肺泡Ⅱ型上皮细胞超微结构变化.结果 各组SP-A平均肺泡阳性细胞数与空白组比较有显著差异(P＜0.01).结论 用气管内滴注脂多糖加烟熏28天法建立的COPD大鼠模型可靠,造模停止两周后模型稳定.
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| 关 键 词： | 嗅鞘细胞 嗅觉神经成纤维细胞 原代培养 免疫细胞化学慢性阻塞性肺疾病 病变特点 实验研究 |
Characteristics of Pathological Changes in Chronic Obstructive Pulmonary Disease (COPD) Rat Model |
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| ZHANG Li,LI Jin-tian,LIU Yong-qi,LI Juan,ZHANG Yi. Characteristics of Pathological Changes in Chronic Obstructive Pulmonary Disease (COPD) Rat Model[J]. Sichuan Journal of Zoology, 2010, 29(2) |
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| Authors: | ZHANG Li LI Jin-tian LIU Yong-qi LI Juan ZHANG Yi |
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| Affiliation: | ZHANG Li,LI Jin-tian,LIU Yong-qi,LI Juan,ZHANG Yi(Gansu College of Traditional Chinese Medcine,Lan zhou 730000,China) |
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| Abstract: | Objective To study the expression of pulmonary surface-active substance related protein A (Surfactant protein A,SP-A) of chronic obstructive pulmonary disease (COPD) rat model and ultrastructural changes in the lung tissue of COPD. Methods The rat model of COPD was established by both intratracheal instillation of lipopolysaccharide (LPS) and passive smoking for 28 days. Observe the general condition of rats,test the expression level of Surfactant protein A (SP-A) by immunohistochemistry,and observe the ult... |
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| Keywords: | COPD characteristics of pathological changes experimental study |
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