Microarray expression profiling of tissues from mice with uniparental duplications of Chromosomes 7 and 11 to identify imprinted genes |
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Authors: | Jonathan D Choi Lara A Underkoffler Joelle N Collins Shannon M Marchegiani Natalie A Terry Colin V Beechey Rebecca J Oakey |
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Institution: | (1) Division of Human Genetics, The Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA, US;(2) MRC Mammalian Genetics Unit, Harwell, Oxfordshire, UK, GB |
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Abstract: | Microarray analysis allows the screening of thousands of identifiable genes in a single experiment. The challenge of this
approach is to combine the new technology with established genetic tools to associate genes with specific biological function.
In this study we have designed a screen to identify imprinted genes from mice with uniparental duplications of proximal Chromosomes
(Chrs) 7 and 11, using microarray analysis. By comparing the expression patterns in embryonic and newborn tissues of maternally
versus paternally inherited proximal Chrs 7 and 11, we have correctly identified four out of five known imprinted genes represented
on a microarray. We have additionally identified two novel imprinted candidate genes as well as a differentially expressed
clone that is a potential downstream target. Interpretation of the microarray data requires careful preparation of age- and
strain-matched samples and attention to detail in tissue dissection technique.
Received: 15 March 2001 / Accepted: 13 June 2001 |
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