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Production of paramylon,a β‐1,3‐glucan,by heterotrophic cultivation of Euglena gracilis on potato liquor
Authors:Bozidar ?antek  Michael Felski  Karl Friehs  Martin Lotz  Erwin Flaschel
Institution:1. Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Pierottijeva, Croatia;2. Faculty of Technology, Bielefeld University, Bielefeld, Germany;3. Emslandst?rke GmbH, Emlichheim, Germany
Abstract:Euglena gracilis is shown to be able to grow on potato liquor as the main medium component leading to an interesting biotechnological product represented by paramylon – a β‐1,3‐glucan – and, at the same time, revaluating an otherwise annoying waste stream of the potato‐starch industry. Paramylon mass fractions of about 75% are obtained for biomass concentrations of 15 g/L during simple batch cultivation under heterotrophic conditions. Supplementation of the growth medium with glucose and the vitamins B1 and B12 are shown to improve growth rate as well as paramylon content. E. gracilis grows best at about 27.5°C without requiring pH control.
Keywords:β  ‐1  3‐glucan  Batch cultivation  Euglena gracilis  Heterotrophic growth  Potato liquor
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